Isaac Progression Guide

0 of 263 doneNext: The Womb

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Stage 1any order

Azazel
Ready
Make 3 deals with the Devil in one run.
Cain
Ready
Hold 55 coins at the same time.
Eve
Ready
Don't pick up any hearts for 2 floors in a row.
Greedier!
Ready
Donate 500 coins to the Greed Donation Machine.
Isaac's Head
Ready
Complete the Boss Rush as Isaac.
Keeper
Ready
Donate 1000 coins to the Greed Donation Machine.
Lazarus
Ready
Have 4 or more soul hearts at the same time.
Lil' Chest
Ready
Defeat Ultra Greed as Isaac.
Magdalene
Ready
Have 7 or more red heart containers at the same time.
Samson
Ready
Complete 2 floors in a row without taking damage.
The Womb
Next up
Defeat Mom.
Reach Depths II and defeat Mom.
Sources:Achievements Womb

Stage 2any order

???
Later
Defeat Mom's Heart 10 times.
After: The Womb
Mom's Heart defeats0 / 10
A Bag of Bombs
Later
Defeat Satan as Cain.
After: Cain, The Womb
A Bag of Pennies
Later
Defeat Isaac as Cain.
After: Cain, The Womb
A Cross
Later
Defeat Isaac as Magdalene.
After: Magdalene, The Womb
Bat Wing
Later
Defeat Ultra Greedier as Azazel.
After: Greedier!, Azazel
Begotten Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Azazel.
After: Azazel, The Womb
Bethany
Later
Defeat Mom's Heart or It Lives! on Hard mode as Lazarus without dying.
After: Lazarus, The Womb
Black Feather
Later
Defeat Ultra Greed as Eve.
After: Eve
Blood Rights
Later
Defeat Satan as Samson.
After: Samson, The Womb
Bloody Lust
Later
Defeat Isaac as Samson.
After: Samson, The Womb
Blue Womb
Later
Defeat Mom's Heart 10 times.
After: The Womb
Mom's Heart defeats0 / 10
Broken Ankh
Later
Defeat Satan as Lazarus.
After: Lazarus, The Womb
Cain's Other Eye
Later
Complete the Boss Rush as Cain.
After: Cain
Censer
Later
Defeat Ultra Greed as Magdalene.
After: Magdalene
Crow Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Eve.
After: Eve, The Womb
Crow Heart
Later
Defeat Ultra Greedier as Eve.
After: Greedier!, Eve
Cute Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Magdalene.
After: Magdalene, The Womb
D1
Later
Defeat Ultra Greedier as Isaac.
After: Greedier!
Daemon's Tail
Later
Defeat Satan as Azazel.
After: Azazel, The Womb
Eden
Later
Complete Chapter 4 (the Womb).
After: The Womb
Eve's Bird Foot
Later
Defeat Isaac as Eve.
After: Eve, The Womb
Eve's Mascara
Later
Complete the Boss Rush as Eve.
After: Eve
Evil Eye
Later
Defeat Ultra Greed as Cain.
After: Cain
Eye of Greed
Later
Defeat Ultra Greedier as Keeper.
After: Greedier!, Keeper
Fighting Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Samson.
After: Samson, The Womb
Glass Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Cain.
After: Cain, The Womb
Glyph of Balance
Later
Defeat Ultra Greedier as Magdalene.
After: Greedier!, Magdalene
Guardian Angel
Later
Defeat Satan as Magdalene.
After: Magdalene, The Womb
Isaac's Tears
Later
Defeat Isaac as Isaac.
After: The Womb
Judas
Later
Defeat Satan.
After: The Womb
Satan defeats0 / 1
Keeper holds Store Key
Later
Defeat Satan as Keeper.
After: Keeper, The Womb
Keeper holds Wooden Nickel
Later
Defeat Isaac as Keeper.
After: Keeper, The Womb
Key Bum
Later
Defeat Ultra Greed as Lazarus.
After: Lazarus
Lazarus' Rags
Later
Defeat Isaac as Lazarus.
After: Lazarus, The Womb
Lilith
Later
Defeat Ultra Greed as Azazel.
After: Azazel
Lost Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Isaac.
After: The Womb
Lusty Blood
Later
Defeat Ultra Greed as Samson.
After: Samson
Maggy's Bow
Later
Complete the Boss Rush as Magdalene.
After: Magdalene
Missing No.
Later
Complete the Boss Rush as Lazarus.
After: Lazarus
Mom's Knife
Later
Defeat Satan as Isaac.
After: The Womb
Plan C
Later
Defeat Ultra Greedier as Lazarus.
After: Greedier!, Lazarus
Rib of Greed
Later
Defeat Ultra Greed as Keeper.
After: Keeper
Sack of Sacks
Later
Defeat Ultra Greedier as Cain.
After: Greedier!, Cain
Samson's Chains
Later
Complete the Boss Rush as Samson.
After: Samson
Satanic Bible
Later
Defeat Isaac as Azazel.
After: Azazel, The Womb
Stem Cell
Later
Defeat Ultra Greedier as Samson.
After: Greedier!, Samson
Sticky Nickels
Later
Complete the Boss Rush as Keeper.
After: Keeper
Super Greed Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Keeper.
After: Keeper, The Womb
The Nail
Later
Complete the Boss Rush as Azazel.
After: Azazel
The Negative
Later
Defeat Satan 5 times.
After: The Womb
Satan defeats0 / 5
The Polaroid
Later
Defeat Isaac 5 times.
After: The Womb
Isaac defeats0 / 5
The Razor
Later
Defeat Satan as Eve.
After: Eve, The Womb
Wrapped Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Lazarus.
After: Lazarus, The Womb

Stage 3any order

???'s Only Friend
Later
Complete the Boss Rush as ???.
After: ???
???'s Soul
Later
Defeat The Lamb as ???.
After: ???, The Womb, The Negative
A Secret Exit
Later
Defeat Hush 3 times.
After: The Womb, Blue Womb
Hush defeats0 / 3
Abaddon
Later
Defeat ??? as Azazel.
After: Azazel, The Womb, The Polaroid
Abel
Later
Defeat The Lamb as Cain.
After: Cain, The Womb, The Negative
Alabaster Box
Later
Defeat The Lamb as Bethany.
After: Bethany, The Womb, The Negative
Angels
Later
Complete Chapter 6 (defeat ??? in the Chest or The Lamb in the Dark Room).
After: The Womb, The Polaroid
Athame
Later
Defeat Hush as Eve.
After: Eve, The Womb, Blue Womb
Beth's Faith
Later
Complete the Boss Rush as Bethany.
After: Bethany
Betrayal
Later
Defeat Hush as Judas.
After: Judas, The Womb, Blue Womb
Black Lipstick
Later
Defeat The Lamb as Eve.
After: Eve, The Womb, The Negative
Blank Card
Later
Defeat Isaac as Eden.
After: Eden, The Womb
Blessed Penny
Later
Defeat ??? as Bethany.
After: Bethany, The Womb, The Polaroid
Blind Rage
Later
Defeat Hush as Samson.
After: Samson, The Womb, Blue Womb
Blood Penny
Later
Defeat ??? as Samson.
After: Samson, The Womb, The Polaroid
Book of Secrets
Later
Defeat Satan as Eden.
After: Eden, The Womb
Book of Virtues
Later
Defeat Isaac as Bethany.
After: Bethany, The Womb
Box of Friends
Later
Defeat Ultra Greed as Lilith.
After: Lilith
Cain's Eye
Later
Defeat ??? as Cain.
After: Cain, The Womb, The Polaroid
Cambion Conception
Later
Defeat ??? as Lilith.
After: Lilith, The Womb, The Polaroid
Celtic Cross
Later
Defeat ??? as Magdalene.
After: Magdalene, The Womb, The Polaroid
Cracked Dice
Later
Defeat Ultra Greed as ???.
After: ???
Curved Horn
Later
Defeat The Lamb as Judas.
After: Judas, The Womb, The Negative
D12
Later
Defeat Hush as Cain.
After: Cain, The Womb, Blue Womb
Dead Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as ???.
After: ???, The Womb
Deep Pockets
Later
Defeat ??? as Keeper.
After: Keeper, The Womb, The Polaroid
Demon Baby
Later
Defeat The Lamb as Azazel.
After: Azazel, The Womb, The Negative
Divine Intervention
Later
Defeat Hush as Bethany.
After: Bethany, The Womb, Blue Womb
Duality
Later
Defeat Ultra Greedier as Lilith.
After: Greedier!, Lilith
Eden's Blessing
Later
Defeat Hush as Eden.
After: Eden, The Womb, Blue Womb
Empty Vessel
Later
Defeat Hush as Lazarus.
After: Lazarus, The Womb, Blue Womb
Eye of Belial
Later
Defeat Ultra Greedier as Judas.
After: Greedier!, Judas
Fart Baby
Later
Defeat Hush as Isaac.
After: The Womb, Blue Womb
Fate
Later
Defeat ??? as ???.
After: ???, The Womb, The Polaroid
Fate's Reward
Later
Defeat Hush as ???.
After: ???, The Womb, Blue Womb
Forget Me Now
Later
Defeat Satan as ???.
After: ???, The Womb
GB Bug
Later
Defeat Ultra Greed as Eden.
After: Eden
Glitch Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Eden.
After: Eden, The Womb
Goat Head Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Lilith.
After: Lilith, The Womb
Guillotine
Later
Defeat Isaac as Judas.
After: Judas, The Womb
Immaculate Conception
Later
Complete the Boss Rush as Lilith.
After: Lilith
Incubus
Later
Defeat Hush as Lilith.
After: Lilith, The Womb, Blue Womb
Judas' Shadow
Later
Complete the Boss Rush as Judas.
After: Judas
Judas' Tongue
Later
Defeat Satan as Judas.
After: Judas, The Womb
Karma
Later
Defeat The Lamb as Keeper.
After: Keeper, The Womb, The Negative
Keeper now holds... A Penny!
Later
Defeat Hush as Keeper.
After: Keeper, The Womb, Blue Womb
Maggy's Faith
Later
Defeat The Lamb as Magdalene.
After: Magdalene, The Womb, The Negative
Maw of the Void
Later
Defeat Hush as Azazel.
After: Azazel, The Womb, Blue Womb
Meconium
Later
Defeat Ultra Greedier as ???.
After: Greedier!, ???
Metronome
Later
Defeat Ultra Greedier as Eden.
After: Greedier!, Eden
Missing Poster
Later
Defeat The Lamb as Isaac.
After: The Womb, The Negative
My Shadow
Later
Defeat Ultra Greed as Judas.
After: Judas
Mysterious Paper
Later
Defeat ??? as Eden.
After: Eden, The Womb, The Polaroid
Mystery Sack
Later
Defeat The Lamb as Eden.
After: Eden, The Womb, The Negative
New Area
Later
Defeat Hush.
After: The Womb, Blue Womb
Hush defeats0 / 1
Pandora's Box
Later
Defeat The Lamb as Lazarus.
After: Lazarus, The Womb, The Negative
Purity
Later
Defeat Hush as Magdalene.
After: Magdalene, The Womb, Blue Womb
Rune Bag
Later
Defeat Isaac as Lilith.
After: Lilith, The Womb
Sacrificial Dagger
Later
Defeat ??? as Eve.
After: Eve, The Womb, The Polaroid
Samson's Lock
Later
Defeat The Lamb as Samson.
After: Samson, The Womb, The Negative
Serpent's Kiss
Later
Defeat Satan as Lilith.
After: Lilith, The Womb
Shadow Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Judas.
After: Judas, The Womb
Soul Locket
Later
Defeat Ultra Greed as Bethany.
After: Bethany
Store Credit
Later
Defeat ??? as Lazarus.
After: Lazarus, The Womb, The Polaroid
Succubus
Later
Defeat The Lamb as Lilith.
After: Lilith, The Womb, The Negative
The D20
Later
Defeat ??? as Isaac.
After: The Womb, The Polaroid
The D6
Later
Defeat Isaac as ???.
After: ???, The Womb
The gate is open!
Later
Defeat The Lamb.
After: The Womb, The Negative
Undefined
Later
Complete the Boss Rush as Eden.
After: Eden
Urn of Souls
Later
Defeat Satan as Bethany.
After: Bethany, The Womb
Vade Retro
Later
Defeat Ultra Greedier as Bethany.
After: Greedier!, Bethany
Wisp Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Bethany.
After: Bethany, The Womb

Stage 4any order

'M
Later
Defeat Mother as Eden.
After: Eden, A Secret Exit
A Strange Door
Later
Defeat Mother.
After: A Secret Exit
Akeldama
Later
Defeat Mother as Judas.
After: Judas, A Secret Exit
Apollyon
Later
Defeat Mega Satan.
After: Angels, The Womb, The Polaroid
Big Baby
Later
Defeat Mega Satan as Lilith.
After: Lilith, Angels, The Womb, The Polaroid
Bird Cage
Later
Defeat Mother as Eve.
After: Eve, A Secret Exit
Black Baby
Later
Defeat Mega Satan as Azazel.
After: Azazel, Angels, The Womb, The Polaroid
Blood Puppy
Later
Defeat Mother as Lilith.
After: Lilith, A Secret Exit
Bloody Crown
Later
Defeat Delirium as Samson.
After: Samson, The Womb, Blue Womb, New Area
Bloody Gust
Later
Defeat Mother as Samson.
After: Samson, A Secret Exit
Blue Baby
Later
Defeat Mega Satan as ???.
After: ???, Angels, The Womb, The Polaroid
Brown Baby
Later
Defeat Mega Satan as Judas.
After: Judas, Angels, The Womb, The Polaroid
Compound Fracture
Later
Defeat Delirium as Lazarus.
After: Lazarus, The Womb, Blue Womb, New Area
Crooked Penny
Later
Defeat Delirium as Keeper.
After: Keeper, The Womb, Blue Womb, New Area
Cry Baby
Later
Defeat Mega Satan as Isaac.
After: Angels, The Womb, The Polaroid
D infinity
Later
Defeat Delirium as Isaac.
After: The Womb, Blue Womb, New Area
Dark Prince's Crown
Later
Defeat Delirium as Azazel.
After: Azazel, The Womb, Blue Womb, New Area
Devil's Crown
Later
Defeat Mother as Azazel.
After: Azazel, A Secret Exit
Dull Razor
Later
Defeat Delirium as Eve.
After: Eve, The Womb, Blue Womb, New Area
Eden's Soul
Later
Defeat Delirium as Eden.
After: Eden, The Womb, Blue Womb, New Area
Eternal D6
Later
Defeat Mother as ???.
After: ???, A Secret Exit
Eucharist
Later
Defeat Delirium as Magdalene.
After: Magdalene, The Womb, Blue Womb, New Area
Euthanasia
Later
Defeat Delirium as Lilith.
After: Lilith, The Womb, Blue Womb, New Area
Glowing Baby
Later
Defeat Mega Satan as Bethany.
After: Bethany, Angels, The Womb, The Polaroid
Green Baby
Later
Defeat Mega Satan as Cain.
After: Cain, Angels, The Womb, The Polaroid
Guppy's Eye
Later
Defeat Mother as Cain.
After: Cain, A Secret Exit
Jacob and Esau
Later
Defeat Mother.
After: A Secret Exit
Keeper's Sack
Later
Defeat Mother as Keeper.
After: Keeper, A Secret Exit
King Baby
Later
Defeat Delirium as ???.
After: ???, The Womb, Blue Womb, New Area
Lil' Baby
Later
Defeat Mega Satan as Eve.
After: Eve, Angels, The Womb, The Polaroid
Long Baby
Later
Defeat Mega Satan as Lazarus.
After: Lazarus, Angels, The Womb, The Polaroid
Meat Cleaver
Later
Defeat Mother as Isaac.
After: A Secret Exit
Noose Baby
Later
Defeat Mega Satan as Keeper.
After: Keeper, Angels, The Womb, The Polaroid
Rage Baby
Later
Defeat Mega Satan as Samson.
After: Samson, Angels, The Womb, The Polaroid
Red Baby
Later
Defeat Mega Satan as Magdalene.
After: Magdalene, Angels, The Womb, The Polaroid
Revelation
Later
Defeat Mother as Bethany.
After: Bethany, A Secret Exit
Shade
Later
Defeat Delirium as Judas.
After: Judas, The Womb, Blue Womb, New Area
Silver Dollar
Later
Defeat Delirium as Cain.
After: Cain, The Womb, Blue Womb, New Area
Star of Bethlehem
Later
Defeat Delirium as Bethany.
After: Bethany, The Womb, Blue Womb, New Area
The Forgotten
Later
Dig up The Forgotten with Mom's Shovel in the Dark Room (a three-part process).
After: The gate is open!, The Womb, The Negative
The Lost
Later
Die in a Sacrifice Room while holding the Missing Poster.
After: Missing Poster
Tinytoma
Later
Defeat Mother as Lazarus.
After: Lazarus, A Secret Exit
Yellow Baby
Later
Defeat Mega Satan as Eden.
After: Eden, Angels, The Womb, The Polaroid
Yuck Heart
Later
Defeat Mother as Magdalene.
After: Magdalene, A Secret Exit

Stage 5any order

-0- Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as The Lost.
After: The Lost, The Womb
A Pound of Flesh
Later
Defeat The Beast as Cain.
After: Cain, The Polaroid, A Strange Door
Astral Projection
Later
Defeat The Beast as Lazarus.
After: Lazarus, The Polaroid, A Strange Door
Birthright
Later
Defeat ??? as Jacob and Esau.
After: Jacob and Esau, The Womb, The Polaroid
Black Rune
Later
Defeat Ultra Greedier as Apollyon.
After: Greedier!, Apollyon
Bone Spurs
Later
Defeat Mother as The Forgotten.
After: The Forgotten, A Secret Exit
Book of the Dead
Later
Defeat Delirium as The Forgotten.
After: The Forgotten, The Womb, Blue Womb, New Area
Bound Baby
Later
Defeat Mega Satan as The Forgotten.
After: The Forgotten, Angels, The Womb, The Polaroid
Brittle Bones
Later
Defeat The Lamb as The Forgotten.
After: The Forgotten, The Womb, The Negative
Brown Nugget
Later
Defeat Ultra Greed as Apollyon.
After: Apollyon
C Section
Later
Defeat The Beast as Lilith.
After: Lilith, The Polaroid, A Strange Door
Candy Heart
Later
Defeat The Beast as Magdalene.
After: Magdalene, The Polaroid, A Strange Door
Cracked Orb
Later
Defeat The Beast as Eve.
After: Eve, The Polaroid, A Strange Door
Dad's Lost Coin
Later
Defeat Ultra Greedier as The Lost.
After: Greedier!, The Lost
Dad's Ring
Later
Defeat Ultra Greedier as The Forgotten.
After: Greedier!, The Forgotten
Damocles
Later
Defeat The Lamb as Jacob and Esau.
After: Jacob and Esau, The Womb, The Negative
Divorce Papers
Later
Complete the Boss Rush as The Forgotten.
After: The Forgotten
Double Baby
Later
Defeat Mom's Heart or It Lives! on Hard mode as Jacob and Esau.
After: Jacob and Esau, The Womb
Empty Heart
Later
Defeat The Beast as Samson.
After: Samson, The Polaroid, A Strange Door
Everything Jar
Later
Defeat The Beast as Eden.
After: Eden, The Polaroid, A Strange Door
Finger Bone
Later
Defeat Ultra Greed as The Forgotten.
After: The Forgotten
Friend Finder
Later
Defeat The Beast as Jacob and Esau.
After: Jacob and Esau, The Polaroid, A Strange Door
Genesis
Later
Defeat Ultra Greedier as Jacob and Esau.
After: Greedier!, Jacob and Esau
Hallowed Ground
Later
Defeat Hush as The Forgotten.
After: The Forgotten, The Womb, Blue Womb
Holy Card
Later
Defeat Delirium as The Lost.
After: The Lost, The Womb, Blue Womb, New Area
Hungry Soul
Later
Defeat The Beast as The Lost.
After: The Lost, The Polaroid, A Strange Door
Hushy
Later
Defeat Hush as Apollyon.
After: Apollyon, The Womb, Blue Womb
Illusion Baby
Later
Defeat Mega Satan as Jacob and Esau.
After: Jacob and Esau, Angels, The Womb, The Polaroid
Inner Child
Later
Defeat Ultra Greed as Jacob and Esau.
After: Jacob and Esau
Isaac's Heart
Later
Defeat Isaac as The Lost.
After: The Lost, The Womb
Jar of Wisps
Later
Defeat The Beast as Bethany.
After: Bethany, The Polaroid, A Strange Door
Jaw Bone
Later
Defeat ??? as The Forgotten.
After: The Forgotten, The Womb, The Polaroid
Keeper's Box
Later
Defeat The Beast as Keeper.
After: Keeper, The Polaroid, A Strange Door
Lil Abaddon
Later
Defeat The Beast as Azazel.
After: Azazel, The Polaroid, A Strange Door
Lil Portal
Later
Defeat Mother as Apollyon.
After: Apollyon, A Secret Exit
Locust of Conquest
Later
Complete the Boss Rush as Apollyon.
After: Apollyon
Locust of Death
Later
Defeat The Lamb as Apollyon.
After: Apollyon, The Womb, The Negative
Locust of Famine
Later
Defeat ??? as Apollyon.
After: Apollyon, The Womb, The Polaroid
Locust of Pestilence
Later
Defeat Satan as Apollyon.
After: Apollyon, The Womb
Locust of Wrath
Later
Defeat Isaac as Apollyon.
After: Apollyon, The Womb
Lost Soul
Later
Defeat Mother as The Lost.
After: The Lost, A Secret Exit
Magic Skin
Later
Defeat Mother as Jacob and Esau.
After: Jacob and Esau, A Secret Exit
Marrow
Later
Defeat Mom's Heart or It Lives! on Hard mode as The Forgotten.
After: The Forgotten, The Womb
Montezuma's Revenge
Later
Defeat The Beast as ???.
After: ???, The Polaroid, A Strange Door
Mort Baby
Later
Defeat Mega Satan as Apollyon.
After: Apollyon, Angels, The Womb, The Polaroid
Options?
Later
Defeat The Beast as Isaac.
After: The Polaroid, A Strange Door
Pointy Rib
Later
Defeat Satan as The Forgotten.
After: The Forgotten, The Womb
Red Stew
Later
Defeat Satan as Jacob and Esau.
After: Jacob and Esau, The Womb
Redemption
Later
Defeat The Beast as Judas.
After: Judas, The Polaroid, A Strange Door
Rock Bottom
Later
Complete the Boss Rush as Jacob and Esau.
After: Jacob and Esau
Slipped Rib
Later
Defeat Isaac as The Forgotten.
After: The Forgotten, The Womb
Smelter
Later
Defeat Mom's Heart or It Lives! on Hard mode as Apollyon.
After: Apollyon, The Womb
Spirit Shackles
Later
Defeat The Beast as The Forgotten.
After: The Forgotten, The Polaroid, A Strange Door
Suplex!
Later
Defeat Delirium as Jacob and Esau.
After: Jacob and Esau, The Womb, Blue Womb, New Area
Sworn Protector
Later
Defeat Hush as The Lost.
After: The Lost, The Womb, Blue Womb
The Body
Later
Defeat ??? as The Lost.
After: The Lost, The Womb, The Polaroid
The D100
Later
Complete the Boss Rush as The Lost.
After: The Lost
The Mind
Later
Defeat Satan as The Lost.
After: The Lost, The Womb
The Soul
Later
Defeat The Lamb as The Lost.
After: The Lost, The Womb, The Negative
The Stairway
Later
Defeat Isaac as Jacob and Esau.
After: Jacob and Esau, The Womb
Vanishing Twin
Later
Defeat Hush as Jacob and Esau.
After: Jacob and Esau, The Womb, Blue Womb
Void
Later
Defeat Delirium as Apollyon.
After: Apollyon, The Womb, Blue Womb, New Area
White Baby
Later
Defeat Mega Satan as The Lost.
After: The Lost, Angels, The Womb, The Polaroid
Worm Friend
Later
Defeat The Beast as Apollyon.
After: Apollyon, The Polaroid, A Strange Door
Zodiac
Later
Defeat Ultra Greed as The Lost.
After: The Lost

Stage 6any order

Apollyon Baby
Later
Earn every completion mark on Hard mode as Apollyon.
After: Void, Black Rune, Locust of Wrath, Locust of Pestilence, Locust of Famine, Locust of Death, Locust of Conquest, Hushy, Brown Nugget, Mort Baby, Smelter, Lil Portal, Worm Friend, Apollyon
Belial Baby
Later
Earn every completion mark on Hard mode as Judas.
After: Curved Horn, Judas' Tongue, Guillotine, Judas' Shadow, Shadow Baby, Betrayal, My Shadow, Brown Baby, Shade, Eye of Belial, Akeldama, Redemption, Judas
Bone Baby
Later
Earn every completion mark on Hard mode as The Forgotten.
After: Marrow, Slipped Rib, Pointy Rib, Jaw Bone, Brittle Bones, Divorce Papers, Hallowed Ground, Finger Bone, Dad's Ring, Book of the Dead, Bound Baby, Bone Spurs, Spirit Shackles, The Forgotten
Buddy Baby
Later
Earn every completion mark on Hard mode as Isaac.
After: Mom's Knife, The D20, Isaac's Head, Isaac's Tears, Lost Baby, Fart Baby, Lil' Chest, Cry Baby, D infinity, D1, Meat Cleaver, Options?
Colorful Baby
Later
Earn every completion mark on Hard mode as Magdalene.
After: A Cross, Guardian Angel, Celtic Cross, Maggy's Faith, Maggy's Bow, Cute Baby, Purity, Censer, Red Baby, Eucharist, Glyph of Balance, Yuck Heart, Candy Heart, Magdalene
Cracked Baby
Later
Earn every completion mark on Hard mode as Eden.
After: Blank Card, Book of Secrets, Mysterious Paper, Mystery Sack, Undefined, Glitch Baby, Eden's Blessing, GB Bug, Yellow Baby, Eden's Soul, Metronome, 'M, Everything Jar, Eden
Dark Baby
Later
Earn every completion mark on Hard mode as Lilith.
After: Incubus, Box of Friends, Big Baby, Rune Bag, Cambion Conception, Serpent's Kiss, Succubus, Immaculate Conception, Goat Head Baby, Euthanasia, Duality, Blood Puppy, C Section, Lilith
Dripping Baby
Later
Earn every completion mark on Hard mode as Lazarus.
After: Missing No., Lazarus' Rags, Broken Ankh, Store Credit, Pandora's Box, Wrapped Baby, Empty Vessel, Key Bum, Long Baby, Compound Fracture, Plan C, Tinytoma, Astral Projection, Lazarus
Godhead
Later
Earn every completion mark on Hard mode as The Lost.
After: Isaac's Heart, The Mind, The Body, The Soul, The D100, -0- Baby, Sworn Protector, Zodiac, White Baby, Holy Card, Dad's Lost Coin, Lost Soul, Hungry Soul, The Lost
Hive Baby
Later
Earn every completion mark on Hard mode as ???.
After: The D6, Forget Me Now, ???'s Soul, Fate, ???'s Only Friend, Dead Baby, Fate's Reward, Cracked Dice, Blue Baby, King Baby, Meconium, Eternal D6, Montezuma's Revenge, ???
Hope Baby
Later
Earn every completion mark on Hard mode as Bethany.
After: Wisp Baby, Book of Virtues, Urn of Souls, Blessed Penny, Alabaster Box, Beth's Faith, Soul Locket, Divine Intervention, Vade Retro, Star of Bethlehem, Glowing Baby, Revelation, Jar of Wisps, Bethany
Picky Baby
Later
Earn every completion mark on Hard mode as Cain.
After: A Bag of Pennies, A Bag of Bombs, Abel, Cain's Eye, Cain's Other Eye, Glass Baby, D12, Evil Eye, Green Baby, Silver Dollar, Sack of Sacks, Guppy's Eye, A Pound of Flesh, Cain
Revenge Baby
Later
Earn every completion mark on Hard mode as Samson.
After: Bloody Lust, Blood Penny, Blood Rights, Samson's Lock, Samson's Chains, Fighting Baby, Blind Rage, Lusty Blood, Rage Baby, Bloody Crown, Stem Cell, Bloody Gust, Empty Heart, Samson
Sale Baby
Later
Earn every completion mark on Hard mode as Keeper.
After: Keeper now holds... A Penny!, Rib of Greed, Noose Baby, Keeper holds Wooden Nickel, Keeper holds Store Key, Deep Pockets, Karma, Sticky Nickels, Super Greed Baby, Crooked Penny, Eye of Greed, Keeper's Sack, Keeper's Box, Keeper
Solomon's Baby
Later
Earn every completion mark on Hard mode as Jacob and Esau.
After: Double Baby, The Stairway, Red Stew, Birthright, Damocles, Rock Bottom, Inner Child, Vanishing Twin, Genesis, Suplex!, Illusion Baby, Magic Skin, Friend Finder, Jacob and Esau
Sucky Baby
Later
Earn every completion mark on Hard mode as Azazel.
After: The Nail, Demon Baby, Satanic Bible, Daemon's Tail, Abaddon, Begotten Baby, Maw of the Void, Black Baby, Dark Prince's Crown, Bat Wing, Devil's Crown, Lil Abaddon, Azazel
Whore Baby
Later
Earn every completion mark on Hard mode as Eve.
After: The Razor, Sacrificial Dagger, Eve's Bird Foot, Black Lipstick, Eve's Mascara, Crow Baby, Athame, Black Feather, Lil' Baby, Dull Razor, Crow Heart, Bird Cage, Cracked Orb, Eve

Stage 7

Mega Mush
Later
Earn every Hard mode completion mark with every non-tainted character.
After: Buddy Baby, Colorful Baby, Picky Baby, Belial Baby, Hive Baby, Whore Baby, Revenge Baby, Sucky Baby, Dripping Baby, Cracked Baby, Godhead, Dark Baby, Sale Baby, Apollyon Baby, Bone Baby, Hope Baby, Solomon's Baby

Stage 1any order

Azazel

Cain

Eve

Greedier!

Isaac's Head

Keeper

Lazarus

Lil' Chest

Magdalene

Samson

The Womb

Stage 2any order

???

A Bag of Bombs

A Bag of Pennies

A Cross

Bat Wing

Begotten Baby

Bethany

Black Feather

Blood Rights

Bloody Lust

Blue Womb

Broken Ankh

Cain's Other Eye

Censer

Crow Baby

Crow Heart

Cute Baby

D1

Daemon's Tail

Eden

Eve's Bird Foot

Eve's Mascara

Evil Eye

Eye of Greed

Fighting Baby

Glass Baby

Glyph of Balance

Guardian Angel

Isaac's Tears

Judas

Keeper holds Store Key

Keeper holds Wooden Nickel

Key Bum

Lazarus' Rags

Lilith

Lost Baby

Lusty Blood

Maggy's Bow

Missing No.

Mom's Knife

Plan C

Rib of Greed

Sack of Sacks

Samson's Chains

Satanic Bible

Stem Cell

Sticky Nickels

Super Greed Baby

The Nail

The Negative

The Polaroid

The Razor

Wrapped Baby

Stage 3any order

???'s Only Friend

???'s Soul

A Secret Exit

Abaddon

Abel

Alabaster Box

Angels

Athame

Beth's Faith

Betrayal

Black Lipstick

Blank Card

Blessed Penny