Challenges

The 45 challenge runs, in game order: what unlocks each one and what beating it gives you.

Pitch Black
challenge
Challenge #1. Goal: Mom.
Available from the start.
Beating it unlocks Rune of Hagalaz
Sources:Challenges Pitch Black
High Brow
challenge
Challenge #2. Goal: Mom.
Available from the start.
Beating it unlocks Rune of Jera
Sources:Challenges High Brow
Head Trauma
challenge
Challenge #3. Goal: Mom.
Available from the start.
Beating it unlocks Rune of Ehwaz
Sources:Challenges Head Trauma
Darkness Falls
challenge
Challenge #4. Goal: Satan.
Unlocked by Darkness Falls (Defeat Mom's Heart 11 times and defeat ??? as Eve)
Beating it unlocks Rune of Dagaz
Sources:Challenges Darkness Falls
The Tank
challenge
Challenge #5. Goal: Mom.
Unlocked by The Tank (Have 7 or more Red Heart containers at one time)
Beating it unlocks Rune of Ansuz
Sources:Challenges The Tank
Solar System
challenge
Challenge #6. Goal: Mom's Heart.
Unlocked by Solar System (Defeat Mom's Heart 3 times)
Beating it unlocks Rune of Perthro
Sources:Challenges Solar System
Suicide King
challenge
Challenge #7. Goal: Isaac.
Unlocked by Suicide King (Defeat Mom's Heart 11 times and unlock Lazarus)
Beating it unlocks Suicide King
Sources:Challenges Suicide King (Challenge)
Cat Got Your Tongue
challenge
Challenge #8. Goal: Mom.
Unlocked by Cat Got Your Tongue (Become Guppy)
Beating it unlocks Rune of Algiz
Sources:Challenges Cat Got Your Tongue
Demo Man
challenge
Challenge #9. Goal: Mom's Heart.
Unlocked by Demo Man (Defeat Mom's Heart 9 times)
Beating it unlocks Chaos Card
Sources:Challenges Demo Man
Cursed!
challenge
Challenge #10. Goal: Mom.
Unlocked by Cursed! (Have 7 or more Red Heart containers at one time)
Beating it unlocks Credit Card
Sources:Challenges Cursed!
Glass Cannon
challenge
Challenge #11. Goal: Satan.
Unlocked by Glass Cannon (Complete The Family Man (challenge #19), defeat Lokii, and unlock Judas and It Lives!)
Beating it unlocks Rules Card
Sources:Challenges Glass Cannon (Challenge)
When Life Gives You Lemons
challenge
Challenge #12. Goal: Mom.
Available from the start.
Beating it unlocks Card Against Humanity
Sources:Challenges When Life Gives You Lemons
Beans!
challenge
Challenge #13. Goal: Mom.
Available from the start.
Beating it unlocks Burnt Penny
Sources:Challenges Beans!
It's in the Cards
challenge
Challenge #14. Goal: Mom.
Available from the start.
Beating it unlocks SMB Super Fan
Sources:Challenges It's in the Cards
Slow Roll
challenge
Challenge #15. Goal: Mom.
Available from the start.
Beating it unlocks Swallowed Penny
Sources:Challenges Slow Roll
Computer Savvy
challenge
Challenge #16. Goal: Mom.
Available from the start.
Beating it unlocks Robo-Baby 2.0
Sources:Challenges Computer Savvy
Waka Waka
challenge
Challenge #17. Goal: Mom.
Available from the start.
Beating it unlocks Death's Touch
Sources:Challenges Waka Waka
The Host
challenge
Challenge #18. Goal: Mom.
Available from the start.
Beating it unlocks Technology.5
Sources:Challenges The Host
The Family Man
challenge
Challenge #19. Goal: Isaac.
Unlocked by The Family Man (Pick up both Key Pieces from the angels in one run and defeat Mom's Heart 11 times)
Beating it unlocks Epic Fetus
Sources:Challenges The Family Man
Purist
challenge
Challenge #20. Goal: Mom's Heart.
Unlocked by Purist (Defeat Mom)
Beating it unlocks Rune of Berkano
Sources:Challenges Purist
XXXXXXXXL
challenge
Challenge #21. Goal: Mom's Heart.
Unlocked by XXXXXXXXL (Defeat Mom)
Beating it unlocks Gold Heart
Sources:Challenges XXXXXXXXL
SPEED!
challenge
Challenge #22. Goal: Mom's Heart.
Unlocked by SPEED! (Defeat Mom)
Beating it unlocks Get out of Jail Free Card
Sources:Challenges SPEED!
Blue Bomber
challenge
Challenge #23. Goal: Satan.
Unlocked by Blue Bomber (Destroy 10 Tinted Rocks and defeat Mom's Heart 11 times)
Beating it unlocks Gold Bomb
Sources:Challenges Blue Bomber
PAY TO PLAY
challenge
Challenge #24. Goal: Isaac.
Unlocked by PAY TO PLAY (Defeat Isaac as Cain)
Beating it unlocks 2 new pills
Sources:Challenges PAY TO PLAY
Have a Heart
challenge
Challenge #25. Goal: Mom's Heart.
Unlocked by Have a Heart (Defeat Mom)
Beating it unlocks 2 new pills
Sources:Challenges Have a Heart
I RULE!
challenge
Challenge #26. Goal: Mega Satan.
Unlocked by I RULE! (Defeat Mega Satan and unlock The Negative)
Beating it unlocks Poker Chip
Sources:Challenges I RULE!
BRAINS!
challenge
Challenge #27. Goal: ???.
Unlocked by BRAINS! (Defeat Isaac 5 times)
Beating it unlocks Stud Finder
Sources:Challenges BRAINS!
PRIDE DAY!
challenge
Challenge #28. Goal: Mom's Heart.
Unlocked by PRIDE DAY! (Defeat Mom)
Beating it unlocks D8
Sources:Challenges PRIDE DAY!
Onan's Streak
challenge
Challenge #29. Goal: Isaac.
Unlocked by Onan's Streak (Unlock Judas and It Lives!)
Beating it unlocks Kidney Stone
Sources:Challenges Onan's Streak
The Guardian
challenge
Challenge #30. Goal: Mom's Heart.
Unlocked by The Guardian (Defeat Mom)
Beating it unlocks Blank Rune
Sources:Challenges The Guardian
Backasswards
challenge
Challenge #31. Goal: Basement I (played in reverse).
Unlocked by Backasswards (Defeat Mega Satan and unlock The Negative)
Beating it unlocks Laz Bleeds More!
Sources:Challenges Backasswards
Aprils Fool
challenge
Challenge #32. Goal: Mom's Heart.
Unlocked by Aprils fool (Defeat Mom)
Beating it unlocks Maggy Now Holds a Pill!
Sources:Challenges Aprils Fool
Pokey Mans
challenge
Challenge #33. Goal: Isaac.
Unlocked by Pokey Mans (Defeat Mom's Heart 11 times)
Beating it unlocks Charged Key
Sources:Challenges Pokey Mans
Ultra Hard
challenge
Challenge #34. Goal: Mega Satan.
Unlocked by Ultra Hard (Defeat Mega Satan and unlock The Negative)
Beating it unlocks Samson Feels Healthy!
Sources:Challenges Ultra Hard
Pong
challenge
Challenge #35. Goal: ???.
Unlocked by PONG (Defeat Isaac 5 times)
Beating it unlocks Greed's Gullet
Sources:Challenges Pong
Scat Man
challenge
Challenge #36. Goal: Mom.
Available from the start.
Beating it unlocks Dirty Mind
Sources:Challenges Scat Man
Bloody Mary
challenge
Challenge #37. Goal: Satan.
Unlocked by Bloody Mary (Unlock Bethany, Blood Bag and It Lives!)
Beating it unlocks Sigil of Baphomet
Sources:Challenges Bloody Mary
Baptism by Fire
challenge
Challenge #38. Goal: Isaac.
Unlocked by Baptism by Fire (Defeat Satan as Bethany, defeat Mom's Heart 11 times, and unlock Maggy's Faith)
Beating it unlocks Purgatory
Sources:Challenges Baptism by Fire
Isaac's Awakening
challenge
Challenge #39. Goal: Mother.
Unlocked by Isaac's Awakening (Defeat Mother)
Beating it unlocks Spirit Sword
Sources:Challenges Isaac's Awakening
Seeing Double
challenge
Challenge #40. Goal: Mom's Heart.
Unlocked by Seeing Double (Defeat Mother)
Beating it unlocks Broken Glasses
Sources:Challenges Seeing Double
Pica Run
challenge
Challenge #41. Goal: Isaac.
Unlocked by Pica Run (Defeat Mom's Heart 11 times and unlock Marbles)
Beating it unlocks Ice Cube
Sources:Challenges Pica Run
Hot Potato
challenge
Challenge #42. Goal: Satan.
Unlocked by Hot Potato (Unlock Tainted Forgotten)
Beating it unlocks The Chariot
Sources:Challenges Hot Potato
Cantripped!
challenge
Challenge #43. Goal: Mom.
Unlocked by Cantripped! (Unlock Tainted Cain)
Beating it unlocks Justice
Sources:Challenges Cantripped!
Red Redemption
challenge
Challenge #44. Goal: Mother.
Unlocked by Red Redemption (Unlock Tainted Jacob)
Beating it unlocks The Hermit
Sources:Challenges Red Redemption
DELETE THIS
challenge
Challenge #45. Goal: ???.
Unlocked by DELETE THIS (Unlock Tainted Eden)
Beating it unlocks Temperance
Sources:Challenges DELETE THIS

Pitch Black

High Brow

Head Trauma

Darkness Falls

The Tank

Solar System

Suicide King

Cat Got Your Tongue

Demo Man

Cursed!

Glass Cannon

When Life Gives You Lemons

Beans!

It's in the Cards

Slow Roll

Computer Savvy

Waka Waka

The Host

The Family Man

Purist

XXXXXXXXL

SPEED!

Blue Bomber

PAY TO PLAY

Have a Heart

I RULE!

BRAINS!

PRIDE DAY!

Onan's Streak

The Guardian

Backasswards

Aprils Fool

Pokey Mans

Ultra Hard

Pong

Scat Man

Bloody Mary

Baptism by Fire

Isaac's Awakening

Seeing Double

Pica Run

Hot Potato

Cantripped!

Red Redemption

DELETE THIS